Question:medium

Vincristine in ultraviolet spectrophotometry \(\lambda_{max}\) value.

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Always use the compound's known $\lambda_{max}$ for most accurate quantitative UV analysis, as this gives the highest molar absorptivity.
Updated On: Jul 14, 2026
  • 217 nm
  • 245 nm
  • 290 nm
  • 360 nm
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The Correct Option is C

Solution and Explanation

Step 1: Understanding the Concept:
Vincristine is a vinca alkaloid, a natural anticancer compound whose UV absorbance comes from its aromatic indole-type ring systems, the parts of the molecule capable of undergoing \( \pi \rightarrow \pi^{*} \) electronic transitions.

Step 2: Key Formula or Approach:
The wavelength at which a molecule absorbs strongly depends on how extended its conjugated system is; more conjugation pushes the absorbance to a longer wavelength. Comparing the candidate wavelengths against how conjugated vincristine's structure actually is narrows down the answer.

Step 3: Detailed Explanation:
Vincristine's structure links two multi-ring alkaloid units, one built around an indole nucleus and the other around a related dihydroindole system, connected through the molecule's core. This combined, moderately extended conjugation places its absorbance in the near-UV region rather than at the shorter wavelengths typical of a single small aromatic ring. Reported spectrophotometric data for vincristine consistently place its \( \lambda_{max} \) at about 290 nm, which fits this level of conjugation without needing the much larger extended system that a value like 360 nm would imply.

Step 4: Final Answer:
Vincristine shows its UV \( \lambda_{max} \) at about 290 nm.
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