Question

To prevent contamination during setup of PCR reaction, which one of these is not important?

• A. Pre- and post-PCR samples should be processed in separate areas
• B. Equipment used for pre- and post-PCR steps should be cleaned after every use
• C. Reagents used for pre- and post-PCR steps should be separated
• D. Adding smaller amounts of Bovine Serum Albumin (BSA) is avoided

Hint:

Focus on physical barriers, proper equipment cleaning, and reagent management to maintain a contamination-free PCR setup.

Answer 1

The Correct Option is D

BSA stabilizes enzymes and improves PCR efficiency; however, it does not prevent contamination. Effective contamination control requires physical separation of pre- and post-PCR areas, thorough equipment cleaning, and dedicated reagents. BSA has no role in contamination prevention.