Given:
- Total magnification: \( M = 20 \)
- Eyepiece magnification: \( M_e = 5 \)
- Microscope length: \( L = 14\,\text{cm} \)
- Least distance of distinct vision: \( D = 25\,\text{cm} \)
Step 1: Calculate Objective Magnification
The objective magnification \( M_o \) is derived from the total magnification \( M \) and the eyepiece magnification \( M_e \) using the formula \( M = M_o \cdot M_e \).
\[ M_o = \frac{M}{M_e} = \frac{20}{5} = 4 \]
Step 2: Objective Magnification Formula
The formula for objective magnification is given by \( M_o = \frac{L - f_e}{f_o} \). Substituting the calculated \( M_o \):
\[ 4 = \frac{14 - f_e}{f_o} \quad \cdots (1) \]
Step 3: Eyepiece Magnification Formula (Relaxed Eye)
For a relaxed eye, the eyepiece magnification \( M_e \) is calculated using \( M_e = 1 + \frac{D}{f_e} \). Solving for \( f_e \):
\[ 5 = 1 + \frac{25}{f_e} \]
\[ \frac{25}{f_e} = 5 - 1 = 4 \]
\[ f_e = \frac{25}{4} = 6.25\,\text{cm} \]
Step 4: Substitute \( f_e \) into Equation (1)
Substitute the calculated value of \( f_e \) into equation (1) to find \( f_o \):
\[ 4 = \frac{14 - 6.25}{f_o} \]
\[ 4 = \frac{7.75}{f_o} \]
\[ f_o = \frac{7.75}{4} = 1.9375\,\text{cm} \]
% Final Answer Statement
Answer:
- Focal length of objective \( f_o = 1.9375\,\text{cm} \)
- Focal length of eyepiece \( f_e = 6.25\,\text{cm} \)