The specific gravity of amyl alcohol (Grade I) at 27$^\circ$ C to be used for fat estimation of milk by Gerber method as per BIS Method is:
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Always run a blank test using distilled water instead of milk to verify the purity of the amyl alcohol.
If any oily layer separates at the top of the column during the blank run, the amyl alcohol is contaminated and must not be used for fat testing.