Question

The principle of fluorescence microscopy is based on:
1. A substance that causes the rotation of polarised light
2. Differences in the optical light path being converted to intensity differences
3. Differences in refractive index being converted into variations in light intensity
4. The absorption of light and its subsequent emission at a longer wavelength

• A. A substance that causes the rotation of polarized light
• B. Differences in the optical light path being converted to intensity differences
• C. Differences in refractive index being converted into variations in light intensity
• D. The absorption of light and its subsequent emission at a longer wavelength

Hint:

Fluorescence microscopy is widely used in biological sciences to study specific structures like proteins, DNA, or organelles by tagging them with fluorescent markers.

Answer 1

The Correct Option is D

Fluorescence microscopy functions by using substances that absorb light (often ultraviolet or blue) at one wavelength and then emit it at a longer, visible wavelength. This characteristic enables specific structures or molecules in a sample to be labeled with fluorescent dyes for detailed visualization.