Question

Study the following steps involved in DNA finger printing-protocol. A) Separation of DNA fragments B) Obtaining DNA C) Denaturation of DNA D) Fragmentation of DNA E) Blotting Arrange the steps in a sequence.

• A. B, A, D, C, E
• B. D, B, A, E, C
• C. B, A, D, E, C
• D. B, D, A, C, E

Hint:

Remember the order: Extract $\rightarrow$ Cut $\rightarrow$ Separate $\rightarrow$ Denature $\rightarrow$ Blot.

Answer 1

The Correct Option is D

Step 1: The goal.
We arrange the steps of DNA fingerprinting in the right laboratory order.

Step 2: Start with the DNA.
First you need the material itself, so you obtain or extract the DNA from the sample. That is step B.

Step 3: Cut it up.
Next the long DNA is cut into many pieces using restriction enzymes. This fragmentation is step D.

Step 4: Sort the pieces.
The fragments are then separated by size using gel electrophoresis. This separation is step A.

Step 5: Make them single-stranded, then blot.
The double-stranded fragments are denatured into single strands (step C) so a probe can later bind. Finally they are transferred onto a membrane by blotting (step E).

Step 6: Conclusion.
The order is B, D, A, C, E, so the answer is "B, D, A, C, E". \[ \boxed{\text{B, D, A, C, E}} \]