Question

Statements related to human insulin are given below: Which statement(s) is/are correct about genetically engineered Insulin?

1. Pro-hormone insulin contain extra stretch of C-peptide
2. A peptide and B-peptide chains of insulin were produced separately in E. coli, extracted and combined by creating a disulphide bond between them.
3. Insulin used for treating Diabetes was extracted from Cattles and Pigs.
4. Pro-hormone insulin needs to be processed for converting into a mature and functional hormone.
5. Some patients develop allergic reactions to the foreign insulin.

Choose the most appropriate answer from the options given below:

• A. (a), (b) and (d) only
• B. (b) only
• C. (c) and (d) only
• D. (c), (d) and (e) only

Answer 1

The Correct Option is B

Step 1: Understanding the Concept:
The production of "Humulin" (human insulin) using recombinant DNA technology addressed supply and allergy issues associated with animal-derived insulin.
Detailed Explanation:
- (a) Correct regarding pro-insulin, but the question asks about genetically engineered insulin (Humulin), which is produced directly in its mature form without C-peptide.
- (b) Correct. Eli Lilly (1983) produced DNA sequences for A and B chains separately, inserted them into plasmids of \(E.\ coli\), and then combined the extracted chains with disulfide bonds.
- (c) and (e) are true for traditional insulin extraction, which caused the allergies mentioned in (e). Genetically engineered insulin solved this.
- (d) Natural pro-insulin needs processing, but engineered insulin is produced already matured to avoid the difficulty of removing C-peptide in bacteria.
Given the specific phrasing "Which statement(s) is/are correct about genetically engineered Insulin?", only (b) describes the actual production process used.
Step 2: Final Answer:
Statement (b) is the correct description of the engineering methodology.