Question

Sequence:
5’ – G$\uparrow$A A T T C – 3’
3’ – C T T A A$\uparrow$G – 5’
(a) Name the restriction enzyme and type of sequence.

Hint:

Palindromic sequences are recognized by restriction enzymes, enabling specific DNA cutting for genetic engineering applications.

Answer 1

• EcoRI enzyme: Cleavage occurs at the specific sequence G$\uparrow$AATTC.
• Sequence characteristic: It is palindromic, meaning it reads the same forwards (5'-3') on one strand as it does backward (3'-5') on its complement.

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