Question

Select the correct statement from the following biotechnological procedures:

• A. The polymerase enzyme joins the gene of interest and the vector DNA.
• B. Gel electrophoresis is used for amplification of a DNA segment.
• C. PCR is used for isolation and separation of the gene of interest.
• D. Plasmid DNA acts as a vector to transfer the piece of DNA attached to it.

Hint:

Plasmids are commonly used as vectors in genetic engineering to introduce new genes into host cells. They can replicate independently and carry foreign genes for expression in host organisms.

Answer 1

The Correct Option is D

Step 1: Statement Analysis:
- Statement (A): "The polymerase enzyme joins the gene of interest and the vector DNA."
This statement is false. Polymerase enzymes facilitate DNA replication but do not ligate (join) the gene of interest to the vector DNA. This function is performed by DNA ligase.
- Statement (B): "Gel electrophoresis is used for amplification of a DNA segment."
This statement is false. Gel electrophoresis separates DNA fragments by size. DNA amplification is achieved through Polymerase Chain Reaction (PCR).
- Statement (C): "PCR is used for isolation and separation of the gene of interest."
This statement is false. PCR amplifies specific DNA sequences. Gene isolation is often performed using restriction enzymes, and separation by size is done via gel electrophoresis.
- Statement (D): "Plasmid DNA acts as a vector to transfer the piece of DNA attached to it."
This statement is true. In genetic engineering, plasmids serve as vectors for introducing foreign DNA into host cells.
Step 2: Conclusion:
Statement (D) is the correct assertion, as plasmid DNA functions as a vector for transporting DNA into a host organism for subsequent research or application.