Question:medium

Select the correct order of the steps involved in Gram staining:
A. Counter staining
B. Crystal violet- Iodine (CV-I)
C. Heat Fixation
D. Decolorizing agent

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Gram Staining Sequence:
1. Heat Fix slide (C) $\to$ 2. Crystal Violet + Iodine (B) $\to$ 3. Alcohol Decolorizer (D) $\to$ 4. Safranin Counterstain (A).
Result: Gram-positive = Purple, Gram-negative = Pink/Red.

Updated On: Jul 28, 2026

B, D, C, A
A, D, B, C
C, A, D, B
C, B, D, A

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The Correct Option is D

Solution and Explanation

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Select the correct order of the steps involved in Gram staining: A. Counter staining B. Crystal violet- Iodine (CV-I) C. Heat Fixation D. Decolorizing agent

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The Correct Option is D

Solution and Explanation

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Questions Asked in CUET (PG) exam

Select the correct order of the steps involved in Gram staining:
A. Counter staining
B. Crystal violet- Iodine (CV-I)
C. Heat Fixation
D. Decolorizing agent