Question:medium

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

Updated On: Nov 26, 2025
  • Hind II, Hind II
  • Hind II, Alu I
  • Hind II, EcoR I
  • Hind II, BamHI
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The Correct Option is A

Solution and Explanation

Formation of a recombinant DNA molecule necessitates the digestion of both vector and source DNA with an identical restriction enzyme. This generates compatible sticky ends that can hybridize, facilitating the insertion of the source DNA into the vector. Conversely, employing distinct restriction enzymes will result in non-complementary sticky ends, precluding the successful construction of the recombinant DNA molecule.

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