Question

Polyethylene glycol method is used for

• A. biodiesel production
• B. seedless fruit production
• C. energy production from sewage
• D. gene transfer without a vector.

Answer 1

The Correct Option is D

To solve this question, we need to understand the role of polyethylene glycol (PEG) in various processes and identify which of the given options correctly describes its application.

Polyethylene glycol (PEG) is a compound that is often utilized in the field of biotechnology and genetic engineering. One of its primary applications is in the process known as gene transfer without a vector. Here, PEG induces the uptake of DNA by cells through a process called PEG-mediated transformation. This is achieved by bringing about cell membrane fusion, allowing the DNA to enter the cell.

Let's evaluate the options:

• Biodiesel production: This involves chemical processes such as transesterification, where PEG is not typically used.
• Seedless fruit production: Generally involves hormonal treatments or genetic modifications, not directly using PEG.
• Energy production from sewage: Involves anaerobic digestion or other biochemical processes, without the direct application of PEG.
• Gene transfer without a vector (Correct Option): PEG is directly involved in mediating gene transfer by facilitating the direct uptake of naked DNA by cells.

Thus, the correct answer is that PEG is used for gene transfer without a vector.