Question:medium
Parents complain that their child was assaulted by a relative and the child has anal pain. On investigation a test shows yellow needle-shaped crystals on adding picric acid. What is the name of the test?
Parents complain that their child was assaulted by a relative and the child has anal pain. On investigation a test shows yellow needle-shaped crystals on adding picric acid. What is the name of the test?
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Picric acid producing yellow spermine crystals names this test.
Updated On: Jun 23, 2026
- Florence test
- Barberio's test
- Acid phosphatase test
- Creatine phosphokinase test
Show Solution
The Correct Option is B
Solution and Explanation
Pattern-match the reagent to the test. Picric acid plus yellow needle-shaped crystals equals Barberio's test, which precipitates spermine as spermine picrate and confirms semen. Tie each seminal test to its reagent and colour to eliminate the rest: Florence test uses iodine and gives brown choline-periodide crystals, so option 1 is wrong because the colour and reagent differ. The acid phosphatase test is an enzymatic colour reaction that relies on the very high acid phosphatase of prostatic fluid, not on picric acid, ruling out option 3. Creatine phosphokinase is a cardiac and muscle enzyme with no role in semen identification, so option 4 is out. The picric-acid, yellow-crystal description fits only Barberio's test, option 2.
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