Question

Paper chromatography is an example of :

• A. Adsorption chromatography
• B. Partition chromatography
• C. Thin layer chromatography
• D. Column chromatography

Answer 1

The Correct Option is B

To determine the correct type of chromatography represented by paper chromatography, we need to understand the basic principle and mechanism of different chromatography techniques.

1. Paper Chromatography: This is a simple and widely used technique for separating components of a mixture. It works on the principle of partition chromatography. In this method, the distribution of substances between the stationary phase (paper) and mobile phase (solvent) is based on the differential partitioning of the components. The substances in the mixture move at different rates due to differences in their solubility and affinity towards the stationary and mobile phases.
2. Explanation of Options:
   1. Adsorption Chromatography: Here, the components are separated based on their adsorption on a solid stationary phase. Examples include column and thin-layer chromatography.
   2. Partition Chromatography: This involves the separation of components based on their partition between a liquid stationary phase and a mobile phase. Paper chromatography is a classic example as the stationary phase is the water trapped in the pores of the paper.
   3. Thin Layer Chromatography (TLC): Similar to paper chromatography, but uses a thin layer of absorbent material such as silica gel on a glass plate. It's based on adsorption rather than partition.
   4. Column Chromatography: Uses solid adsorbents packed in a column and separates compounds based on their adsorption affinity.
3. Conclusion: Based on the principles discussed, paper chromatography is best categorized as partition chromatography, because it involves the partitioning of analytes between the water (stationary phase) and the mobile phase (solvent).