Question

Match the technique with its application. 

• A. A-1, B-2, C-3, D-4
• B. A-4, B-3, C-2, D-1
• C. A-1, B-3, C-2, D-4
• D. A-2, B-1, C-4, D-3

Hint:

Use the mnemonic \textbf{SNOW DROP} to remember blotting targets:
\textbf{S}outhern = \textbf{D}NA
\textbf{N}orthern = \textbf{R}NA
\textbf{O} = \textbf{O} (placeholder)
\textbf{W}estern = \textbf{P}rotein

Answer 1

The Correct Option is A

To solve the question, we need to match each technique in Column I with its correct application in Column II.

Let's go through each option:

1. A. PCR - DNA Amplification: PCR (Polymerase Chain Reaction) is used for amplifying DNA sequences. Thus, it matches with 1 in Column II.
2. B. Western blot - Protein Detection: Western blotting is a technique used to detect specific proteins in a sample. Therefore, it matches with 2 in Column II.
3. C. Northern blot - RNA Detection: Northern blotting is used to detect RNA sequences. Hence, it corresponds to 3 in Column II.
4. D. Southern blot - DNA Detection: Southern blotting is a method used for detecting specific DNA sequences. Thus, it matches with 4 in Column II.

From this analysis, the correct matching is:

A-1, B-2, C-3, D-4

This confirms that the correct answer is indeed: A-1, B-2, C-3, D-4.