Question

Match sequencing methods with key feature. 

• A. A-1, B-2, C-3, D-4
• B. A-2, B-1, C-4, D-3
• C. A-3, B-4, C-1, D-2
• D. A-4, B-3, C-2, D-1

Hint:

Key associations for sequencing tech: Sanger = ddNTPs/termination. Illumina = Reversible terminators/fluorescence SBS. Nanopore = Current/electrical changes. Pyrosequencing = Pyrophosphate/Luciferase light flash.

Answer 1

The Correct Option is A

To solve this question, we need to match each sequencing method with its key feature.

1. Sanger sequencing: This method utilizes the chain termination technique. Modified nucleotides (dideoxynucleotides) are incorporated, terminating the DNA strand elongation at specific bases. Thus, A-1.
2. Illumina sequencing: This is characterized by sequencing by synthesis. It utilizes reversible dye terminators to allow for the reading of DNA sequences bases through cycles of synthesis. Therefore, B-2.
3. Nanopore sequencing: This technique is known for electrical signal detection. As DNA strands pass through a nanopore, changes in the electrical current are measured, allowing sequence determination. So, C-3.
4. Pyrosequencing: This method detects the release of pyrophosphate released during nucleotide incorporation, which is transformed into light signals, thus involving light detection. Hence, D-4.

The correct matching is:

A-1, B-2, C-3, D-4

This matches with the option indicating these correlations, confirming the correct answer.