Question

Match List-I with List-II.

List-I	List-II
A. Transformation	I. Restriction enzyme
B. Cloning site	II. Transfer DNA to host bacteria
C. Selection	IV. Antibiotic
D. Ori	III. Replication

Choose the correct answer from the options given below :

• A. A-IV, B-I, C-III, D-II
• B. A-II, B-I, C-IV, D-III
• C. A-I, B-II, C-IV, D-III
• D. A-III, B-IV, C-II, D-I

Hint:

• "Ori" is the direct initiator of Replication, allowing you to link D with III.

• Commonly used antibiotic resistance genes for selection in E. coli vectors include those for ampicillin, chloramphenicol, tetracycline, and kanamycin.

Answer 1

The Correct Option is B

Step 1: Understand the matching task.
We must pair each rDNA term in List-I with its correct description in List-II.
Step 2: Match A, Transformation.
Transformation is the process of introducing foreign DNA into a host bacterium, so A pairs with II, Transfer DNA to host bacteria.
Step 3: Match B, Cloning site.
A cloning site is a spot on the vector cut by a restriction enzyme to insert foreign DNA, so B pairs with I, Restriction enzyme.
Step 4: Match C, Selection.
Selection picks out recombinant cells using a selectable marker such as an antibiotic resistance gene, so C pairs with IV, Antibiotic.
Step 5: Match D, Ori.
The origin of replication (Ori) starts DNA replication and controls copy number, so D pairs with III, Replication.
Step 6: Combine and conclude.
The combination is A-II, B-I, C-IV, D-III, which is option 2.
\[ \boxed{\text{A-II, B-I, C-IV, D-III}} \]