Question

Match List-I with List-II:

List-I	List-II
(A) Alkaline phosphatase	(i) Removes phosphate group at 5’ end of DNA
(B) DNA Polymerase	(ii) Nick translation
(C) SI Nuclease	(iii) Cleaves ssDNA
(D) DNase I	(iv) Cleaves DNA

Choose the correct answer from the options given below:

• (A) - (IV), (B) - (III), (C) - (II), (D) - (I)
• (A) - (I), (B) - (III), (C) - (II), (D) - (IV)
• (A) - (I), (B) - (II), (C) - (IV), (D) - (III)
• (A) - (III), (B) - (IV), (C) - (I), (D) - (II)

Hint:

Alkaline phosphatase removes phosphate groups, DNA polymerase is involved in nick translation, S1 nuclease is involved in single stranded DNA cleavage, and DNase I degrades DNA randomly.

Answer 1

The Correct Option is A

Enzyme Functions:
• Alkaline phosphatase: Removes 5’ phosphate groups from DNA and RNA.
• DNA polymerase: Engages in nick translation.
• S1 Nuclease: Cleaves single-stranded DNA.
• DNase I: Cleaves DNA randomly.
Consequently, the correct associations are: (A) - (IV), (B) - (III), (C) - (II), (D) - (I)