Question

(iv) Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?

Hint:

Insertional inactivation is a biosafe method for identifying recombinants without relying on antibiotics.

Answer 1

- Recombinant colonies are directly identifiable via insertional inactivation due to observable phenotypic changes resulting from gene disruption (e.g., loss of color or enzyme activity).
- This method enhances biosafety by eliminating the need for antibiotics.