Yes, there is a clear difference in how the poet describes the two roads.
(i) In stanzas two and three:
In these stanzas, the poet shows that both roads are almost the same. He says they are equally grassy and worn about the same, and that neither road had been used much. This means there is no real difference between the two roads at the time of choice.
(ii) In the last two lines of the poem:
In the last two lines, the poet creates a difference between the roads by saying he took the one “less travelled by.” Here, the road becomes a symbol of a unique and independent choice. The poet suggests that this choice made all the difference in his life, even though earlier both roads were actually similar.