Question

In gel filtration chromatography, which molecules elute first?

• A. Small molecules
• B. Highly charged molecules
• C. Hydrophobic molecules
• D. Large molecules

Hint:

In gel filtration chromatography, \[ \boxed{ \text{Large molecules} \rightarrow \text{Elute first} } \] \[ \boxed{ \text{Small molecules} \rightarrow \text{Elute later} } \] because small molecules enter the pores of the gel beads.

Answer 1

The Correct Option is D