Question

In Eijkmans test, inoculum from positive PCT tubes is transferred to MacConkey's broth tubes and incubated at ________.

• A. 44.5 $\pm$ 0.2$^\circ$C for 24 $\pm$ 2h
• B. 42.0 $\pm$ 0.5$^\circ$C for 24 $\pm$ 2h
• C. 44.5 $\pm$ 0.2$^\circ$C for 48 $\pm$ 2h
• D. 42.0 $\pm$ 0.2$^\circ$C for 48 $\pm$ 2h

Hint:

Remember the diagnostic temperature for fecal coliforms:
- Standard coliforms = $37^\circ\text{C}$.
- Fecal coliforms (E. coli) = $44.5^\circ\text{C}$ in a water bath.
The temperature must be controlled precisely within $\pm 0.2^\circ\text{C}$.

Answer 1

The Correct Option is A