Question

In DNA fingerprinting, which of the following techniques is used to amplify specific regions of DNA for analysis?

• A. Polymerase Chain Reaction (PCR).
• B. Gel Electrophoresis.
• C. DNA Sequencing.
• D. Southern Blotting.

Hint:

PCR is a vital tool in molecular biology, allowing the amplification of specific DNA regions for applications like fingerprinting and diagnostics.

Answer 1

The Correct Option is A

Step 1: PCR's Function in DNA Fingerprinting.
Polymerase Chain Reaction (PCR) is a method for amplifying specific DNA segments, generating millions of DNA fragment copies. These amplified fragments are then used in DNA fingerprinting to identify genetic markers.

Step 2: Analysis of Alternative Techniques.
Gel Electrophoresis (Option B): Separates DNA fragments by size; it does not amplify DNA.

DNA Sequencing (Option C): Determines the nucleotide order of DNA; it is not used for amplification in fingerprinting.

Southern Blotting (Option D): Detects specific DNA sequences; it does not involve amplification.

Step 3: Final Determination.
PCR is the essential technique for amplifying DNA regions in fingerprinting. \[ \therefore \text{The correct answer is: Polymerase Chain Reaction (PCR).]