Question

In Capillary Electrophoresis, for a representative 50 cm capillary column operated at 30,000 V, typical net mobility for a 10 min migration time is about $2\times10^{-8}\ \text{m}^2\text{s}^{-1}\text{V}^{-1}$. Calculate the number of theoretical plates for an ion having diffusion coefficient $D = 1\times10^{-9}\ \text{m}^2\text{s}^{-1}$.

• A. 3,000
• B. 30,000
• C. 3,00,000
• D. 1,50,000

Hint:

In CE: $N = \dfrac{\mu_{\text{net}} \cdot V}{2D}$. High voltage and low diffusion coefficient give more theoretical plates (better separation).

Answer 1

The Correct Option is C