The genotype of a black seed plant can be determined via a test cross. Black seeds can result from a homozygous dominant (BB) or heterozygous (Bb) genotype. To distinguish between these, cross the black seed plant with a homozygous recessive plant (bb), which exhibits white seeds. The rationale is as follows:
Consequently, crossing the black seed plant with a homozygous recessive (bb) plant allows for the determination of the black seed plant's genotype based on the progeny's phenotypes. This experimental procedure is termed a test cross. The appropriate cross is therefore with bb.