Question

(iii) A cloning vector does not have a selectable marker. How will it affect the process of cloning?

Hint:

Selectable markers simplify recombinant identification, making cloning faster and more efficient.

Answer 1

- The absence of a selectable marker hinders the differentiation of recombinant from non-recombinant cells.
- The necessity for supplementary screening techniques, such as PCR or DNA sequencing, leads to increased time and expense.