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(iii) A cloning vector does not have a selectable marker. How will it affect the process of cloning?

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Selectable markers simplify the identification of recombinant DNA in host cells.
Updated On: Jan 13, 2026
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Solution and Explanation

Selectable markers are crucial for identifying recombinant cells. Their absence leads to: 1. Increased complexity: Reliance on alternative methods like PCR or sequencing would escalate costs and lengthen timelines. 2. Higher error rate: Non-recombinant cells might proliferate more than recombinant ones, complicating selection. 3. Importance of markers: For instance, antibiotic resistance genes simplify identification by permitting only successfully transformed cells to grow. Consequently, omitting markers significantly complicates cloning workflows and diminishes efficiency.% Quick tip
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