Question

Hybridoma technique is used to obtain

• A. purified antibodies
• B. purified antigens
• C. purified lymphocytes
• D. purified macrophages

Hint:

Hybridoma = B lymphocyte plus myeloma cell, made to mass produce one specific antibody.

Answer 1

The Correct Option is A

The hybridoma technique is a lab method that fuses two different cells together, and the question asks what final product this method is built to supply.

1. purified antibodies: A hybridoma is made by fusing an antibody making B lymphocyte with a myeloma cell that can divide forever. The resulting hybrid cell line keeps churning out the exact same antibody that its parent B lymphocyte was making, giving a limitless, pure supply of one antibody, known as a monoclonal antibody. This is the true purpose of the technique.
2. purified antigens: Antigens are the substances given beforehand to trigger the immune response and make the B lymphocyte in the first place. They are the starting trigger, not the output of the fusion technique.
3. purified lymphocytes: A lymphocyte is only one of the two cells fused together to build the hybridoma. Getting more lymphocytes is not the goal of the process.
4. purified macrophages: Macrophages play no role in building a hybridoma and are not related to this technique at all.

Since the whole design of cell fusion is to keep a B lymphocyte's antibody-making ability alive forever in a dividing cell line, the product obtained is pure antibody.

Let's summarize:

• Hybridoma equals a B lymphocyte, which makes antibody, fused with a myeloma cell, which divides endlessly.
• The output is monoclonal antibody, meaning one pure, unlimited antibody type.

So the hybridoma technique is used to obtain purified antibodies.