Question:hard

How do you arrange the correct sequence of the following procedural steps for early pregnancy diagnosis in cow?
(A) Do the insemination in adult dairy cows.
(B) Administer a small dose (2.5 mg or 0.5 ml) of Dinoprost (Lutalyse) intravenously through the ear vein.
(C) Observe critically the teat of the animal for alveolar milk to start flowing about one minute later.
(D) After 18 to 22 days and 3 hours prior to the evening milking, place the teat cannula in the left fore-teat and leave it for milk flow from the teat cistern till it ceases.
Choose the correct answer from the options given below which describe the correct sequential steps for early pregnancy diagnosis in cow.

Show Hint

Breeding comes first, then draining the cistern, then the hormone injection, then observing the response.
  • (A), (B), (C), (D)
  • (A), (C), (D), (B)
  • (A), (D), (C), (B)
  • (A), (D), (B), (C)
Show Solution

The Correct Option is D

Solution and Explanation

Think of this test as a hormone response check rather than a memorised list. First the cow must be bred, so insemination (A) always comes first. About three weeks later, the milk cistern is drained through a cannula so that no old milk is left to confuse the result, so (D) comes next. Only after the cistern is empty does it make sense to inject the Dinoprost dose (B), because the test measures whether this injection triggers a fresh oxytocin driven milk let down. The final step is watching the teat for that let down within about a minute (C), which tells whether the corpus luteum, and so the pregnancy, is still active. $\boxed{(A), (D), (B), (C)}$
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