The hair perforation test is used classically to distinguish between two common Trichophyton species based on their ability to penetrate hair in vitro.
The hair perforation test distinguishes dermatophytes by their capacity to actively invade (perforate) hair shafts in vitro, producing wedge-shaped perforations perpendicular to the hair shaft.
Key results to remember:
Organism
Hair Perforation Test
$\textit{T. mentagrophytes}$
Positive
$\textit{T. rubrum}$
Negative
$\textit{M. audouinii}$
Negative
$\textit{E. floccosum}$
Does not infect hair
The primary clinical use of this test is to differentiate $\textit{T. mentagrophytes}$ from $\textit{T. rubrum}$, as both are common causes of tinea pedis and tinea unguium but require different clinical approaches.