Question

F. Skoog observed that callus proliferated from the internodal segments of tobacco stem when auxins was supplied with one of the following except :

• A. Extract of Vascular tissues
• B. Coconut milk
• C. Abscisic acid
• D. Yeast Extract

Answer 1

The Correct Option is C

F. Skoog’s research with tobacco callus demonstrated that callus growth necessitates auxin, in addition to substances from vascular tissues, yeast extract, or coconut milk. These substances provide cytokinins, which, when combined with auxin, are vital for cell division and development. Coconut milk is notably abundant in cytokinins (specifically kinetin). Abscisic acid (ABA), a plant hormone associated with growth suppression, dormancy, and stress adaptation, does not stimulate cell division or proliferation in callus cultures.