Question

Explain how the sterilization of the growth media is achieved in the laboratory.

Hint:

Remember the different methods of sterilisation, and the principles on which they work.

Answer 1

Autoclaving: Growth media is commonly sterilized using autoclaving. This involves heating the media to 121°C under 15 psi pressure for 15-20 minutes within an autoclave, effectively eliminating microbes and spores.

Membrane Filter Sterilization: Media can also be sterilized by filtration. The media is passed through a membrane with fine pores (0.2 or 0.22 micrometer), physically removing microbes and rendering the media sterile.