To determine the correct enzyme-substrate pair, we must first understand the roles of the given enzymes and substrates:
Based on the above analysis, the correct enzyme-substrate pair from the options given is Rennin - Casein.
Conclusion: Rennin is the enzyme that acts on the substrate casein, justifying why this pairing is correct.
| List I | List II | ||
| A | Lipase | I | Peptide bond |
| B | Nuclease | II | Ester bond |
| C | Protease | III | Glycosidic bond |
| D | Amylase | IV | Phosphodiester bond |
The initial step in the digestion of milk in humans is carried out by the enzyme \( \text{Renin (Rennin)} \).