Question

Choose correct statements about two-dimensional electrophoresis. A. It is a combination of IEF and SDS-PAGE.
B. It separates proteins of identical molecular weight that differ in pI.
C. It can't separate proteins with similar pI values but differ in molecular weight.
D. Thousands of cellular proteins can be resolved with this technique.
E. Microscopy can be used to identify individual protein spots in IEF.
Choose the correct answer from the options given below :

• A. \(B, C\) only
• B. \(A, C, E\) only
• C. \(A, B, D\) only
• D. \(C, D\) only

Hint:

Remember: \[ \text{2D Electrophoresis} = \text{IEF} + \text{SDS-PAGE} \]
• First dimension \(\rightarrow\) Separation by \(pI\)
• Second dimension \(\rightarrow\) Separation by molecular weight \[ \text{Widely used in proteomics studies} \]

Answer 1

The Correct Option is C