The biolistic gun, also known as a gene gun, introduces DNA into plant cells by propelling DNA-coated microprojectiles at high speed. This method is crucial for genetic engineering, facilitating the modification of plant cells that are resistant to other transformation techniques. It is extensively applied in the development of genetically modified crops, the investigation of gene expression, and the generation of transgenic plants. Its effectiveness and adaptability render it essential for agricultural and biotechnological research, particularly when working with plants possessing robust cell walls.% Quick tip