As per Food Safety and Standards (Food Product Standards and Additives Regulations, 2011), the RP-HPLC method shall be used for checking for the presence of ____ in ghee.
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Beta-sitosterol is the primary plant sterol (phytosterol) used as a marker to detect vegetable fat adulteration in dairy products. Since animal fats contain only cholesterol, detecting any phytosterol indicates adulteration.