Question

Arrange the steps of agarose gel electrophoresis in correct order :
A. Preparation of agarose gel
B. Visualization under UV light
C. Loading of DNA samples
D. Migration of DNA fragments
E. Application of electric current
Choose the correct answer from the options given below :

• A. \(C, D, A, B, E\)
• B. \(A, B, C, D, E\)
• C. \(B, D, E, A, C\)
• D. \(A, C, E, D, B\)

Hint:

Remember: \[ \text{Smaller DNA fragments migrate faster in agarose gel} \] Sequence of electrophoresis: \[ \text{Prepare Gel} \rightarrow \text{Load Sample} \rightarrow \text{Run Current} \rightarrow \text{Migration} \rightarrow \text{Visualization} \]
• DNA is visualized using UV light after staining.

Answer 1

The Correct Option is D