Question

Arrange the steps in southern blotting in correct order :
A. Transfer of DNA to nitrocellulose membrane
B. Detection of hybridized probe
C. Restriction digestion of DNA
D. Hybridization with labelled probe
E. Gel electrophoresis
Choose the correct answer from the options given below :

• A. \(A, C, D, E, B\)
• B. \(E, C, A, B, D\)
• C. \(C, E, A, D, B\)
• D. \(B, C, D, E, A\)

Hint:

Remember the sequence: \[ \text{Digest} \rightarrow \text{Separate} \rightarrow \text{Transfer} \rightarrow \text{Hybridize} \rightarrow \text{Detect} \]
• Southern blot \(\rightarrow\) DNA
• Northern blot \(\rightarrow\) RNA
• Western blot \(\rightarrow\) Protein

Answer 1

The Correct Option is C