Question

Arrange the following steps involved in Gram staining technique in correct sequence:
A. Preparation of smear and fixation,
B. Decolourization with alcohol,
C. Addition of iodine,
D. Counter staining, E. Differential feature becomes apparent. Choose the correct answer from the options given below:

• A. A, C, B, E, D
• B. A, B, C, D, E
• C. C, A, B, D, E
• D. B, C, A, E, D

Hint:

In Gram staining, decolourization is the most critical step for differentiating Gram-positive and Gram-negative bacteria.

Answer 1

The Correct Option is A