Question

Arrange the following centrifugal speeds in an order to be used when a cell homogenate is to be separated into various fractions. A. \(1000\, g\)
B. \(80{,}000\, g\)
C. \(1{,}50{,}000\, g\)
D. \(20{,}000\, g\)
E. \(60{,}000\, g\)
Choose the correct answer from the options given below :

• A. \(A, D, E, B, C\)
• B. \(A, E, C, D, B\)
• C. \(C, B, E, D, A\)
• D. \(E, D, B, A, C\)

Hint:

Remember: \[ \text{Lower speed} \rightarrow \text{Larger organelles} \] \[ \text{Higher speed} \rightarrow \text{Smaller particles} \] Typical order in differential centrifugation: \[ \text{Nuclei} \rightarrow \text{Mitochondria} \rightarrow \text{Microsomes} \rightarrow \text{Ribosomes} \]

Answer 1

The Correct Option is A