Question

After binding to the two strands of the double helix DNA, where specifically does the restriction enzyme act to cut the two strands of DNA? Write the specific term used for the specific nucleotide sequences of DNA recognised by a restriction endonuclease.

Hint:

Recognition sequences in DNA are palindromic, enabling restriction enzymes to bind and cut at precise locations.

Answer 1

Restriction enzymes cleave double-stranded DNA at specific recognition sites, which are usually palindromic sequences. These sites determine where the enzyme cuts the DNA, producing either sticky or blunt ends. The precise nucleotide sequence that a restriction endonuclease recognizes is termed the recognition sequence.