Question

A spectrophotometer is used to measure the concentration of a molecule in a solution based on Beer's law using cuvette of path length 10mm and detectable absorbance of 0.01. The molar absorptivity of the solution is $10^4$ l/mol-m. The minimum concentration of the solution that can be detected is

• A. $1\mu\text{mol/l}$
• B. $24\mu\text{mol/l}$
• C. $5\mu\text{mol/l}$
• D. $10\mu\text{mol/l}$

Hint:

Always double-check unit dimensions when working with analytical instrumentation questions.
In almost all standard bio-instrumentation applications, the cuvette path length is normalized to \( 1\text{ cm} \) (which equals \( 10\text{ mm} \)).
Keeping this standard benchmark in mind makes calculations very straightforward.

Answer 1

The Correct Option is A