(a) Draw a schematic diagram of the cloning vector pBR322 and label (1) Bam HI site (2) gene for ampicillin resistance (3) ‘ori’ (4) ‘rop’ gene.

Question

Read the passage given below and answer the questions that follow: In recombinant DNA technology, restriction enzymes are used as they recognize and cut DNA within a specific recognition sequence. BamHI is one such restriction enzyme which binds at the recognition sequence 5’-G↓GATCC-3’ and cleaves this sequence between G and G on each strand, whereas AluI binds at the recognition sequence 5’-AG↓CT-3’ and cleaves these sequences between G and C on each strand.
If Alu I is used to cut the given DNA strand, how many DNA fragments would be formed? Write the sequence of each fragment formed with its polarity.
\begin{figure}[H] \centering \includegraphics[width=0.8\textwidth]{Screenshot 2025-01-17 115424.png} \caption{DNA sequence representation with complementary base pairing.} \label{fig:dna_sequence} \end{figure}

Answer 1

- Bam HI site: Used for cloning.
- Ampicillin resistance gene: Functions as a selectable marker.
- ‘Ori’: The origin of replication, critical for plasmid replication.
- ‘Rop’ gene: Controls the plasmid copy number.

(a) Draw a schematic diagram of the cloning vector pBR322 and label (1) Bam HI site (2) gene for ampicillin resistance (3) ‘ori’ (4) ‘rop’ gene.

Show Hint

Solution and Explanation

Top Questions on Ecosystem

Questions Asked in CBSE Class XII exam